Substrate specificity of isolated Drosophila tafazzin. A, Apparent acyl specificity of purified Drosophila tafazzin (MBP-TAZ, 2.5 μg) was determined by 5-min incubations with [14C]LPC (5 μM, 0.05 μCi) and various molecular species of PC (50 μM). B, Apparent head group specificity of purified Drosophila tafazzin (MBP-TAZ, 2.5 μg) was determined by 5-min incubations with [14C]LPC (5 μM, 0.05 μCi) and linoleoyl species of various phospholipids (50 μM). Recovery of radioactivity was measured in PC. Bar graphs represent means ± SEM (n=3).