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. Author manuscript; available in PMC: 2010 Apr 1.
Published in final edited form as: Biochim Biophys Acta. 2009 Jan 21;1791(4):314–320. doi: 10.1016/j.bbalip.2009.01.004

Fig. 5.

Fig. 5

Effect of tafazzin on the molecular composition of CL. A, Acyl exchange between PC and CL was determined in the presence of MBP-TAZ (2.5 μg), 10 μM [14C]acyl labeled PC, and 60 μM CL. The amount of [14C]CL was measured after 30 min. Bar graph represents means ± SEM (n=3). B, CL-MLCL transacylation was measured in a mixture containing MBP-TAZ (2.5 μg), 0.01 μCi [14C]MLCL and 20 nmol CL in a volume of 0.2 mL. C, Molecular composition of CL was determined by fluorescence-HPLC in control Sf9 cells and in Sf9 cells expressing Drosophila tafazzin (TAZ). In each chromatogram, the fluorescence yield is plotted against the retention time from 30 to 50 min.