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Partitioning of glycosphingolipids between coexisting fluid phases in GPMVs. GPMVs were generated from cells pre-labeled with Rh-DOPE and either A488-CTB (A), A488-AA4 (B) or bodipy-C5-GM1 (C). A488-CTB bound to GM1 (A), A488-AA4 bound to acetylated GD1b (B), and acyl chain-labeled Bodipy-C5-GM1 (C) prefer the Lo-like phase and shows partitioning complementary to Rh-DOPE in GPMVs. (Scale bars, 5 μm.)
