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. Author manuscript; available in PMC: 2009 May 9.
Published in final edited form as: Biochim Biophys Acta. 2007 Sep 12;1778(1):20–32. doi: 10.1016/j.bbamem.2007.08.028

Figure 5.

Figure 5

Partitioning of GPI-anchored proteins between coexisting fluid phases in GPMVs. GPMVs were generated from cells transiently expressing YFP-GPI or mYFP-GL-GPI and subsequently labeled with Rh-DOPE. Confocal images of equatorial (A) or tangential sections (B) through GPMVs comparing the partitioning preference of GPI-anchored proteins with the Ld-marker Rh-DOPE. (A) YFP-GL-GPI is strongly enriched in the Lo-like phase in GPMVs while Rh-DOPE labels the Ld-like phase, and (B) mYFP-GL-GPI partitions preferentially into the Lo-like phase, complementary to the Rh-DOPE labeled Ld-like phase. (Scale bars, 5 μm.)