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Partitioning of fluorescently labeled inner leaflet-associated proteins in phase-separated GPMVs. GPMVs were labeled either with A488-CTB or Rh-DOPE after isolation from cells transiently expressing PH-dsRed or flotillin-2-EGFP. (A) PH-dsRed shows partitioning complementary to Lo marker A488-CTB, and, (B) flotillin-2-EGFP cosegregates with the Ld marker Rh-DOPE. (Scale bars, 5 μm.)
