Skip to main content
. Author manuscript; available in PMC: 2009 May 9.
Published in final edited form as: Biochem Biophys Res Commun. 2007 Aug 8;362(1):56–62. doi: 10.1016/j.bbrc.2007.07.163

Figure 1.

Figure 1

RalBP1 was precipitated from 293T cells transiently transfected with RalBP1-HA and detected directly with coomassie blue staining or indirectly through anti-HA antibodies, anti-RalBP1 antibodies or anti phosphor-threonine (pThr) antibodies. Two solubilization protocols were evaluated, RIPA buffer or TritonX-100 containing buffer. Antigen was detected before precipitation in whole cell lysate (WCL) and in whole cell lysate after precipitation (WAI). The precipitate was boiled and loaded (IP), and enrichments and purification were observed as depicted. (*) Antibody fragments.