Skip to main content
PMC home page
Journal of Clinical Microbiology logoLink to Journal of Clinical Microbiology
. 1990 Jul;28(7):1575–1579. doi: 10.1128/jcm.28.7.1575-1579.1990

Use of immunoblotting to detect Aspergillus fumigatus antigen in sera and urines of rats with experimental invasive aspergillosis.

B Yu 1, Y Niki 1, D Armstrong 1
PMCID: PMC267991  PMID: 2199519

Abstract

Immunoblotting was used to detect Aspergillus fumigatus antigen in sera and urines of immunosuppressed rats experimentally infected with A. fumigatus. Organisms were administered by both intravenous and intratracheal injections. Intravenously infected rats developed disseminated aspergillosis, but intratracheally infected rats developed pulmonary disease only. Fungal cultures of blood and urine samples from infected rats were negative. In the urines of intravenously infected rats, antigen was detected 24 to 48 h after infection; in the urines of intratracheally infected animals, antigen was detected on days 4 to 5 after infection. Antigen in serum was detected later than antigen in urine was. Following sodium dodecyl sulfate-polyacrylamide gel electrophoresis and immunoblotting of serum and urine samples, the most strongly reacting antigenic materials were found in the 88-, 40-, 27-, and 20-kilodalton regions. These dominant antigens appeared to be the same as those of control antigens prepared from A. fumigatus grown in vitro. Rabbit antiserum to Aspergillus filtrate antigen was found to be more immunoreactive than antiserum to mycelial or conidial antigen. No mycelium-specific antigens were detected.

Full text

PDF
1576

Images in this article

1577Image
on p.1577
1577Image
on p.1577
1577Image
on p.1577
1578Image
on p.1578
1578Image
on p.1578

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Aisner J., Wiernik P. H., Schimpff S. C. Treatment of invasive aspergillosis: relation of early diagnosis and treatment to response. Ann Intern Med. 1977 May;86(5):539–543. doi: 10.7326/0003-4819-86-5-539. [DOI] [PubMed] [Google Scholar]
  2. DeGregorio M. W., Lee W. M., Linker C. A., Jacobs R. A., Ries C. A. Fungal infections in patients with acute leukemia. Am J Med. 1982 Oct;73(4):543–548. doi: 10.1016/0002-9343(82)90334-5. [DOI] [PubMed] [Google Scholar]
  3. Dupont B., Huber M., Kim S. J., Bennett J. E. Galactomannan antigenemia and antigenuria in aspergillosis: studies in patients and experimentally infected rabbits. J Infect Dis. 1987 Jan;155(1):1–11. doi: 10.1093/infdis/155.1.1. [DOI] [PubMed] [Google Scholar]
  4. Fisher B. D., Armstrong D., Yu B., Gold J. W. Invasive aspergillosis. Progress in early diagnosis and treatment. Am J Med. 1981 Oct;71(4):571–577. doi: 10.1016/0002-9343(81)90208-4. [DOI] [PubMed] [Google Scholar]
  5. Huskinson J., Stepick-Biek P., Remington J. S. Detection of antigens in urine during acute toxoplasmosis. J Clin Microbiol. 1989 May;27(5):1099–1101. doi: 10.1128/jcm.27.5.1099-1101.1989. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Hyde F. W., Johnson R. C., White T. J., Shelburne C. E. Detection of antigens in urine of mice and humans infected with Borrelia burgdorferi, etiologic agent of Lyme disease. J Clin Microbiol. 1989 Jan;27(1):58–61. doi: 10.1128/jcm.27.1.58-61.1989. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Marier R., Smith W., Jansen M., Andriole V. T. A solid-phase radioimmunoassay for the measurement of antibody to Aspergillus in invasive aspergillosis. J Infect Dis. 1979 Nov;140(5):771–779. doi: 10.1093/infdis/140.5.771. [DOI] [PubMed] [Google Scholar]
  8. Matthews R., Burnie J. P., Fox A., Tabaqchali S. Immunoblot analysis of serological responses in invasive aspergillosis. J Clin Pathol. 1985 Nov;38(11):1300–1303. doi: 10.1136/jcp.38.11.1300. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Meyer R. D., Young L. S., Armstrong D., Yu B. Aspergillosis complicating neoplastic disease. Am J Med. 1973 Jan;54(1):6–15. doi: 10.1016/0002-9343(73)90077-6. [DOI] [PubMed] [Google Scholar]
  10. Patterson T. F., Miniter P., Ryan J. L., Andriole V. T. Effect of immunosuppression and amphotericin B on Aspergillus antigenemia in an experimental model. J Infect Dis. 1988 Aug;158(2):415–422. doi: 10.1093/infdis/158.2.415. [DOI] [PubMed] [Google Scholar]
  11. Phillips P., Radigan G. Antigenemia in a rabbit model of invasive aspergillosis. J Infect Dis. 1989 Jun;159(6):1147–1150. doi: 10.1093/infdis/159.6.1147. [DOI] [PubMed] [Google Scholar]
  12. Reiss E., Lehmann P. F. Galactomannan antigenemia in invasive aspergillosis. Infect Immun. 1979 Jul;25(1):357–365. doi: 10.1128/iai.25.1.357-365.1979. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Sabetta J. R., Miniter P., Andriole V. T. The diagnosis of invasive aspergillosis by an enzyme-linked immunosorbent assay for circulating antigen. J Infect Dis. 1985 Nov;152(5):946–953. doi: 10.1093/infdis/152.5.946. [DOI] [PubMed] [Google Scholar]
  14. Schaefer J. C., Yu B., Armstrong D. An aspergillus immunodiffusion test in the early diagnosis of aspergillosis in adult leukemia patients. Am Rev Respir Dis. 1976 Mar;113(3):325–329. doi: 10.1164/arrd.1976.113.3.325. [DOI] [PubMed] [Google Scholar]
  15. Schmitt H. J., Bernard E. M., Häuser M., Armstrong D. Aerosol amphotericin B is effective for prophylaxis and therapy in a rat model of pulmonary aspergillosis. Antimicrob Agents Chemother. 1988 Nov;32(11):1676–1679. doi: 10.1128/aac.32.11.1676. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Shaffer P. J., Medoff G., Kobayashi G. S. Demonstration of antigenemia by radioimmunoassay in rabbits experimentally infected with Aspergillus. J Infect Dis. 1979 Mar;139(3):313–319. doi: 10.1093/infdis/139.3.313. [DOI] [PubMed] [Google Scholar]
  17. Talbot G. H., Weiner M. H., Gerson S. L., Provencher M., Hurwitz S. Serodiagnosis of invasive aspergillosis in patients with hematologic malignancy: validation of the Aspergillus fumigatus antigen radioimmunoassay. J Infect Dis. 1987 Jan;155(1):12–27. doi: 10.1093/infdis/155.1.12. [DOI] [PubMed] [Google Scholar]
  18. Weiner M. H. Antigenemia detected by radioimmunoassay in systemic aspergillosis. Ann Intern Med. 1980 Jun;92(6):793–796. doi: 10.7326/0003-4819-92-6-793. [DOI] [PubMed] [Google Scholar]
  19. Weiner M. H., Coats-Stephen M. Immunodiagnosis of systemic aspergillosis. I. Antigenemia detected by radioimmunoassay in experimental infection. J Lab Clin Med. 1979 Jan;93(1):111–119. [PubMed] [Google Scholar]
  20. Wilson E. V., Hearn V. M., Mackenzie D. W. Evaluation of a test to detect circulating Aspergillus fumigatus antigen in a survey of immunocompromised patients with proven or suspected invasive disease. J Med Vet Mycol. 1987 Dec;25(6):365–375. [PubMed] [Google Scholar]
  21. Young R. C., Bennett J. E., Vogel C. L., Carbone P. P., DeVita V. T. Aspergillosis. The spectrum of the disease in 98 patients. Medicine (Baltimore) 1970 Mar;49(2):147–173. doi: 10.1097/00005792-197003000-00002. [DOI] [PubMed] [Google Scholar]
  22. de Repentigny L., Boushira M., Ste-Marie L., Bosisio G. Detection of galactomannan antigenemia by enzyme immunoassay in experimental invasive aspergillosis. J Clin Microbiol. 1987 May;25(5):863–867. doi: 10.1128/jcm.25.5.863-867.1987. [DOI] [PMC free article] [PubMed] [Google Scholar]

Articles from Journal of Clinical Microbiology are provided here courtesy of American Society for Microbiology (ASM)

RESOURCES