Figure 6.

BDNF and pTrkB immunoreactivity in neurogenic regions. Optical sections were taken at 40× magnification of coronal slices of the mouse SVZ (a), RMS (b), and selOB (c), immunolabeled for BDNF (red) and counterstained with DAPI (4,6,diamidino-2-phenylindole; blue). Scale bars, 50 μm. Middle, Representative coronal sections of the mouse RMS taken at 20× magnification and coimmunolabeled for pTrkB receptor (d), BDNF (e), and merged (f). Included is an inset from confocal imaging of this same section showing BDNF to be highly localized surrounding pTrkB positive cells. Scale bars, 100 μm. g, Bottom, Representative immunoblots from intraperitoneal Westerns blotted for pTrkB and subsequently for total TrkB proteins in SVZ tissue, as well as quantification of percentage difference in the percentage activated TrkB corrected for total TrkB levels. Quantification is presented as percentage difference from wild-type density, with error bars depicting SEM. Asterisk indicates statistically significant difference (Student's t test, *p < 0.05).