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. Author manuscript; available in PMC: 2009 May 10.
Published in final edited form as: Mol Carcinog. 2008 May;47(5):326–337. doi: 10.1002/mc.20389

Figure 4.

Figure 4

DLC-1 expression reduces RhoA activity at the leading edge of cellular protrusions. MEFs stably expressing a RhoA biosensor were transiently-transfected with mCherry-tagged DLC-1 and plated on fibronectin. (A) Time-lapse images of RhoA activity during cellular protrusion. Regions of intense RhoA activity are shown in red. Scale bar, 10 μm. (B) Quantification of RhoA activity at varying distances from the edge of the cell. RhoA activity was quantified along 120 line-scans drawn perpendicular to the edge of 8 mCherry control cells and 103 line-scans perpendicular to the edge of 8 mCherry DLC-1 cells. Data points represent the average ± SD (C) mCherry DLC-1 is expressed at focal adhesions in the MEFs. Expression of the mCherry fluorescence is shown for representative control and DLC-1 cells.