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. Author manuscript; available in PMC: 2009 May 11.
Published in final edited form as: J Biol Chem. 2006 Sep 13;281(46):35478–35486. doi: 10.1074/jbc.M607204200

FIGURE 2. The engineered disulfide bond prevents the conversion to latent AT.

FIGURE 2

A, the conversion to latent AT can be followed by monitoring loss of inhibitory activity with time. Under the conditions used, control AT was converted to the latent form with a half-life of 18 h, whereas the V317C/T401C variant was fully resistant to the conformational transition. B, successful formation of the disulfide bond was further verified by solving the crystal structure of the V317C/T401C variant (yellow) in the context of the active/latent dimer. The RCL and parts of strands 1C and 2C are shown with surrounding electron density (contoured at 1 × the root mean squared deviation of the map, 1σ). The structure of native wild-type AT is superimposed for comparison (cyan), and the engineered disulfide bond is shown in green.