TABLE 2.

Biochemical properties of AT variants

Antithrombin	Pentasaccharide binding (Kd)			Penta	Rates of inhibition (kapp)
	I = 0.15		I = 0.30		Thrombin		Factor Xa
		nm				m−1 s−1
Control	3.5 ± 0.3		26 ± 0.3	−	7.3 ± 0.4 × 103		4.9 ± 0.5 × 103
				+	1.10 ± 0.06 × 104		7.0 ± 0.3 × 105
V317C/T401C	7.1 ± 1.1		62 ± 0.3	−	1.47 ± 0.04 × 103		9.6 ± 0.7 × 102
				+	6.9 ± 0.2 × 103		1.86 ± 0.09 × 105
E237A	1.8 ± 0.1		15 ± 3	−	1.4 ± 0.1 × 104		7.6 ± 0.5 × 103
				+	1.4 ± 0.2 × 104		6.4 ± 0.4 × 105
E237K	2.0 ± 0.1		13.5 ± 0.7	−	2.3 ± 0.2 × 104		1.4 ± 0.1 × 104
				+	1.9 ± 0.2 × 104		7.5 ± 0.3 × 105