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. 2009 May 19;4(5):e5603. doi: 10.1371/journal.pone.0005603

Figure 3. Cyclic expression of Sprouty4 depends on Hes7.

Figure 3

(A–C) Expression pattern of Sprouty4 in Hes7-null or Lfng-null mutant embryos. In E10.5 Hes7-/- mutant embryos, Sprouty4 is expressed in similar tissues as seen in wild-type embryos (A). All Hes7-/- mutant embryos display a uniform expression in the PSM (n = 24) (B). Lfng-/- mutant embryos display various expression patterns in the PSM (n = 5) (C). Scale bars: A, 1 mm; B, 200 µm; C, 100 µm. (D–F) Promoter analysis of Sprouty4. (D) The 1.7 kb Sprouty4 promoter, which contains 3 consensus sequences of N-box and 3 consensus sequences of E-box, was inserted upstream of luciferase. (E) Hes7 protein was specifically detected by Western blotting with anti-Hes7 antibody in the Hes7 transfected cells. β-actin were detected in Hes7 transfected and control cells. (F) Luciferase activity under the control of the Sprouty4 promoter was not significantly suppressed in the presence of Hes7 (lane 2,3). However, the activity induced by bFGF (lane 4) was inhibited in the presence of Hes7 (lane 5,6). Luciferase activity under the control of the Sprouty4 promoter with mutations of N-box and E-box was not significantly suppressed in the presence of Hes7 again (lane 8,9). The activity induced by bFGF (lane 10) was not significantly inhibited in the presence of Hes7 (lane 11,12).