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. 2009 May 19;4(5):e5603. doi: 10.1371/journal.pone.0005603

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Hayashi et al.

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

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(A–C) Whole mount embryos show expression of Sprouty4 in the PSM, telencephalon T), midbrain-hindbrain boundary (M), heart and brachial arch primordia (H) in lateral views of 6 somite stage zebrafish embryos. Lateral view and anterior to the top. (D–F) The expression patterns of Sprouty4 in the zebrafish PSM do not vary among embryos. Dorsal view and anterior to the top. The stained embryos were flat mounted by removing the yolk (G, H). Red arrowheads indicate the anterior limit of the Sprouty4 positive region. Scale bars: A, G, 200 µm.