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Expression of MFG-E8 mRNA in the liver during postnatal development. Liver samples from E20 and P0–5–10–15 were collected. Total RNA was extracted as described in the Methods. The MFG-E8 RNA transcript was quantified by real-time RT-PCR at each time point. The transcript abundance was normalized to the amount present in the liver at E20. Data are representatives of 3 independent experiments and displayed as their means ± SD. **P<0.0001 compared with E20.
