Fig. 7.

Expression of CDP-choline pathway enzymes in NIH-3T3 fibroblasts transduced with ATF6α(1-373). NIH-3T3 cells were transduced with empty vector (black bars) or ATF6α(1-373) (gray bars) retroviruses and harvested at 48 hours post-transduction. Total RNA was prepared and the relative levels of expression of the CK α and β isoforms (Chkα and Chkβ), the CCT isoforms α, β2 and β3, and Chpt1 and Cept1 were measured by quantitative real-time PCR using gene-specific primers and probes. The amount of target RNA was normalized to endogenous Gapdh as a reference. The mean ± s.e.m. of triplicate determinations is plotted and is representative of two independent experiments.