Fig. 8.

Schematic relationship between the proteome and enzome of the fertilization envelope. (A) The biochemical proteome, adapted, with permission, from Wong and Wessel (Wong and Wessel, 2006b), and its link to the enzyme regulatory network that is needed to assemble the fertilization envelope. No evidence implicates β-1,3-glucanase as a contributor to the formation or maturation of the fertilization envelope. Short broken lines indicate ionic protein binding; unbroken black lines indicate dityrosine crosslinking by ovoperoxidase; long broken green lines indicate transamidation by the transglutaminases; solid arrows indicate positive activation. (B) The binding proteome mapped onto the vitelline layer during fertilization envelope assembly. Protein symbols are as in A. (C) Chronology of enzyme activity during fertilization envelope assembly. Colorized enzymes are as in A. See text for details. Adapted, with permission, from Wong and Wessel (Wong and Wessel, 2008). (D) Time course of enzyme activities related to inter-protein crosslinking measured after fertilization occurs. Separate curves for transamidation (Battaglia and Shapiro, 1988), hydrogen peroxide production (Wong et al., 2004) and dityrosine crosslinking (Wong and Wessel, 2008) are taken or extrapolated from previous work, and are plotted against the surface pH changes measured previously (Smith et al., 2002). Images of eggs and zygotes are to temporal scale with the x-axis, showing that fertilization envelope assembly is completed 1 minute after fertilization. Shaded regions predict events that occur within the fertilization envelope at a given time.