The concentration of cGMP in follicle-enclosed oocytes decreases in
response to LH. (A,B) The YFP/CFP emission ratio from
follicle-enclosed mouse oocytes containing cGi500, before and after injecting
1 mM cGMP, for no treatment (basal) (A) and for ∼1 hour after applying LH
(B). (C) Percentage change in the YFP/CFP emission ratio for cGi500 in
response to injection of 1 mM cGMP, for follicle-enclosed oocytes with or
without LH treatment. The percentage change in YFP/CFP emission ratio was
13.9±1.4 (n=21) for no LH, 30.1±0.6 (n=25) for
1-1.3 hours LH, and 30.6±0.5 (n=9) for 5 hours LH.