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. 2009 May 8;136(11):1869–1878. doi: 10.1242/dev.035238

Fig. 7.

Fig. 7.

Decreasing cGMP by injecting follicle-enclosed oocytes with the catalytic domain of PDE9A decreases cAMP. (A) Determination of the percentage change in the YFP/CFP emission ratio for Epac2-camps300 in a follicle-enclosed mouse oocyte that was injected with 180 μg/ml PDE9A catalytic domain and then ∼1 hour later injected with 1 mM cAMP. The cAMP injection lowered the YFP/CFP emission ratio by 31.2±0.7% (n=6). (B) cAMP concentrations after injection of PDE9A, or BSA control (200 μg/ml), determined using the calibration curve in Fig. 4C. cAMP concentrations for basal and 1-1.4 hour LH conditions, from Fig. 4C, are shown for comparison.