Fig. 9.

cGMP concentrations in follicle-enclosed oocytes treated with reagents that affect gap junction closure, and in follicle-enclosed oocytes and whole follicles in response to LH. (A,B) The YFP/CFP emission ratio from follicle-enclosed mouse oocytes containing the cGMP sensor cGi500, before and after injecting 1 mM cGMP, for follicles treated with 100 μM CBX (A), or LH plus 10 μM U0126 (B). (C) cGMP concentrations in follicle-enclosed oocytes after various treatments (see text), as determined from measurements as in A,B and the calibration curve in Fig. 6C. The values for basal and 1-1.3 hour LH conditions are from Fig. 6C. Oocytes had not undergone NEBD at the time of the measurements. (D) cGMP concentrations in whole follicles with or without treatment for 1 hour with LH (mean±s.e.m. for n independent assays). Basal, 2300±310 nM (n=8); 1 hour LH, 85±14 nM (n=8).