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. Author manuscript; available in PMC: 2009 May 11.
Published in final edited form as: J Neural Eng. 2007 Apr 4;4(2):130–145. doi: 10.1088/1741-2560/4/2/013

Figure 1.

Figure 1

Expression of immature hematopoietic and neural antigens in the HUCBmnf non-adherent fraction. Panel A: representative photomicrographs of replated non-adherent HUCBmnf cells that express immature hematopoietic and non-hematopoietic markers after 14 DIV. (a) 29 ± 3% of CD34 positive (+) small round cells (red) were found. In addition, many CD56 (NCAM) immunoreactive cells were also found (b). (c) While 58 ± 5% of the non-adherent cells still expressed common leukocyte antigen CD45 (green), 93 3% of the adherent HUCBmnf cells were positive for CD45, suggesting that they maintained their hematopoietic heritage. A number of cells in the non-adherent HUCBmnf were negative for this antigen. (d) The immature neural marker, nestin, (red) as well as early neuronal marker TuJ1 ((e), red) were expressed. (f) The majority of the replated non-adherent HUCBmnf cells (93 ± 6%) were immunopositive for the stem cell marker Oct-4 (green). DAPI (blue) counterstaining was used to visualize all nucleated cells in the culture. Scale bar = 50 μm (a), (b), (d), (e) and (f), and 20 μm in (c). Panel B: after 14 DIV, the expression of immature hematopoietic markers (CD117 and CD34) and the neural marker, nestin, was greater in the non-adherent HUCBmnf cells compared to the adherent cells while the hematopoietic markers (CD45 and CD56) were predominantly found in adherent cells. Significant differences between the two groups were assessed by Student's t-test (*p < 0.05, **p < 0.001).