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. Author manuscript; available in PMC: 2009 May 11.
Published in final edited form as: J Neural Eng. 2007 Apr 4;4(2):130–145. doi: 10.1088/1741-2560/4/2/013

Figure 3.

Figure 3

Proliferative capacity of non-adherent HUCBmnf after 10 DIV with or without growth factors. Panel A: (a) brightfield photomicrograph of HUCBmnf cultured for 10 DIV without growth factors. The culture in (b) was treated with hEGF and hbFGF. BrdU+ cells were found in both the non-treated (c) and growth factor treated (d) cultures. The insets in (c) and (d) show BrdU labeled cells at higher magnification. Scale bar = 50 μm in (a)-(d), and 20 μm in inset of (c) and (d). Panel B: the proliferative capacity of the non-adherent cells tended to be greater than in the adherent HUCBmnf cells at 14 DIV (p = 0.06). Panel C: within the nonadherent fraction, treatment of the cultures with growth factors increased BrdU incorporation into the cells. Significant differences between the two groups were assessed by Student's t-test (*p < 0.05, **p < 0.001).