Table II.
Fraction missing | Tilt Series equivalent |
Ignoring effect of missing wedge |
Accounting for missing wedge |
---|---|---|---|
.00 | ±90.0° | 90, −180, 180 | 90, −180, 180 |
.10 | ±81.0° | 90, −180, 180 | 90, −180, 130 |
.20 | ±72.0° | 90, −180, 280 | 90, 0, 0 |
.25 | ±67.5° | 90, −180, 280 | 90, −170, 10 |
.30 | ±63.0° | 0, 0, 90 | 80, 0, −150 |
.40 | ±54.0° | 0, 0, 90 | 80, 170, −40 |
.50 | ±45.0° | 10, 0, −180 | 20, 40, −60 |
orientation searches for 5 other different pairs of starting orientations of GroEL, and results for all 6 ribosome orientation searches, are in Supplementary Data