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. Author manuscript; available in PMC: 2010 Mar 1.
Published in final edited form as: J Neuroimmune Pharmacol. 2008 Nov 25;4(1):103–115. doi: 10.1007/s11481-008-9139-x

Figure 4. Effect of FoxD3 and YY1 silencing on OTK18 promoter regulation.

Figure 4

The cell line was co-transfected with pJ6 empty plasmid (negative control) or pre-verified siRNA against human FoxD3 (A) or YY1 (B), pGL3 series [−884/+1, −805/+1, −777/+1, −743/+1(FoxD3*), or −725/+1], and pTK-RL. PGL3-[−743/+1(FoxD3*)] reporter plasmid has null mutation in FoxD3 response element. The cells were subjected to dual-luciferase assay 2 days after the reporter gene transfection. * denotes p<0.05 vs. pJ6 transfected group of the same pGL3 series transfection as determined by ANOVA and Newman Keuls post-hoc, respectively. N.C. represents no statistical changes between groups.