Table 2.
Substrate | Enzyme activity (μM/min) | Normalized activity (%) |
---|---|---|
FPP | 0.765 ± 0.007 | 100 |
Compound 5 | 0.104 ± 0.006 | 14 |
Irradiated Compound 5 | 0.681 ± 0.012 | 89 |
Compound 9 | 0.058 ± 0.001 | 7.6 |
Irradiated Compound 9 | 0.557 ± 0.006 | 73 |
When present, FPP and/or caged FPP were each at 10 μM. Given the rate of photolysis, the irradiated samples would be expected to contain 10 μM free FPP and side products. The CAAX peptide substrate is N-Dansyl-GCVIA