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. Author manuscript; available in PMC: 2009 May 11.
Published in final edited form as: Cell. 2008 Jan 25;132(2):208–220. doi: 10.1016/j.cell.2007.12.029

Figure 5. Transposon End Cleavage Can Be Re-Directed.

Figure 5

(A) SDS-PAGE gel showing the results of cleavage assays. Upon cleavage of an 8-mer that spans the LE cleavage site, TnpA (green oval) becomes covalently attached to four nt and can be resolved from unmodified TnpA. TnpA cleaves LE and RE cleavage substrates (cs) only when the appropriate 5′ extension is added to IP sequences. No covalent complex is formed with the Y127F active site mutant. (B) Modification of cleavage specificity. Shown are the results of LE cleavage assays using LE26 and LEcs, and two variants.