Figure 4.
ELISA analysis for cochlin immunoreactivity. ELISA analysis using MOCAS medium from the indicated samples at the indicated time points was performed with 1 μg of protein and hCochlin-3 antibody. Co-chlin secretion into the medium after TGFβ2 treatment was at a low level for 2 to 3 days and then increased with time of incubation. Control (without TGFβ2 treatment) samples showed absorbance values similar to those at baseline and are not depicted. Each bar represents the mean ± SEM from three independent experimental readings and was found to be significantly different from 0.0 at each time point by the one-sample t-test (*P < 0.05).