Figure 2.

Dependence of the CaM–(RL–VS) interaction on Ca²⁺. (Top) Diagram depicting placement of RL–VS within the α_1C sequence. (A) Binding of [³⁵S]RL–VS (Upper) to GST-CaM (Lower). (B) Average of four experiments, two at 2.5 mM total EGTA and two at 5 mM total EGTA. For quantification, the [³⁵S]RL–VS bound to GST-CAM was counted in a liquid scintillation counter. Data were normalized to [³⁵S]RL–VS bound at 1.6 μM Ca²⁺.