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. Author manuscript; available in PMC: 2009 Nov 1.
Published in final edited form as: Mol Cancer Ther. 2008 Nov;7(11):3586–3597. doi: 10.1158/1535-7163.MCT-08-0653

Figure 4. Anti-EGFRvIII siRNA reduces the invasiveness of glioma cells.

Figure 4

A, light microscopy (×40) images of the density of invaded U87Δ cells on the third day following control electroporation. Invasiveness was evaluated by a Matrigel-based cell invasion assay. Representative of three independent experiments done at 72 h post-electroporation. Cells were fixed and stained with crystal violet. Bar, 300 μm. B, light microscopy (×40) images of the density of invaded U87Δ cells on the third day following electroporation with anti-EGFRvIII siRNA. Invasiveness was evaluated by a Matrigel-based cell invasion assay. Bar, 300 μm. C, quantification of invaded U87Δ cells following electroporation with and without anti-EGFRvIII siRNA. This was achieved by dissolving U87Δ cells in NP-40 solution and performing spectrophotometry.