Table 2.
Tex binding affinities from gel mobility shift assays.
| Protein | Kda (uM) | Percent Binding Relative to WT |
|---|---|---|
| 25 nt ssRNA | ||
| WT Tex | 0.21 ± 0.05 | 100% |
| ΔS1 Tex | ndb | < 0.1% |
| R718E | 28.4 ± 11.4 | 0.74 ± 0.30 % |
| F668D/F671D | 13.7 ± 0.7 | 1.53 ± 0.08 % |
| F668D | 15.5 ± 5.5 | 1.35 ± 0.48 % |
| F671D | 9.8 ± 1.7 | 2.14 ± 0.37 % |
| H683E | 10.7 ± 3.5 | 1.96 ± 0.64 % |
| 25 bp dsRNA | ||
| WT Tex | 4.2 ± 1.47 | 100% |
| ΔS1 Tex | nd | < 0.1% |
| R718E | 46.9 ± 6.3 | 8.96 ± 1.20 % |
| F668D/F671D | 21.2 ± 11.5 | 19.8 ± 10.6 % |
| 25 bp dsDNA | ||
| WT Tex | 3.7 ± 0.15 | 100% |
| ΔS1 Tex | nd | < 0.1% |
| R718E | 48.3 ± 5.2 | 7.66 ± 0.82 % |
| F668D/F671D | 20.4 ± 3.8 | 18.1 ± 3.38 % |
| 25 bp dsDNA | ||
| WT Tex | 5.1 ± 1.5 | 100% |
| ΔS1 Tex | nd | < 0.1% |
| R718E | 118.7 ± 10.7 | 4.30 ± 0.39 % |
| F668D/F671D | 55.3 ± 6.0 | 9.22 ± 1.00 % |
a
Values represent the average Kd from multiple experiments (2 ≤ n ≤ 9) ± standard error
b
No binding detected