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. 2009 Feb 4;29(5):1486–1495. doi: 10.1523/JNEUROSCI.3882-08.2009

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Copyright © 2009 Society for Neuroscience 0270-6474/09/291486-10$15.00/0

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Figure 6. — Immunocytochemistry showing mislocalization of M-opsin in irbp ^−/− photoreceptors. A, M-opsin immunoreactivity in a section of distal retina from a wild-type mouse. B, M-opsin immunoreactivity in a distal retina section from an irbp ^−/− mouse. C, M-opsin immunoreactivity in a distal retina section from an irbp ^−/− mouse treated with 9-cis-RAL. D, Representative differential interference contrast (DIC) image of a similar retina section from an irbp ^−/− mouse showing cell layers: sclera and choroid (Sc/Ch), outer and inner segment layer (OS/IS), outer nuclear layer (ONL), OPL. E, Estimates of M-opsin and S-opsin percentage-mislocalization derived by dividing OPL immunofluorescence by the sum of OPL and OS immunofluorescence. Note the restoration of M-opsin and S-opsin mislocalization in irbp ^−/− mice after administering 9-cis-RAL.