Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2008 Dec 10;71(3):730–747. doi: 10.1111/j.1365-2958.2008.06559.x

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

Journal compilation © 2008 Blackwell Publishing

Re-use of this article is permitted in accordance with the Creative Commons Deed, Attribution 2.5, which does not permit commercial exploitation.

PMC Copyright notice

Fig. 1 — Upper panel: Immunoblot analysis of PilY1 in P. aeruginosa cell lysates. From left to right: PilY1-immunoreactive signals from planktonic cells of P. aeruginosa PAO1 (126.6 kDa), lane 1; TBCF10839 (126.3 kDa), lane 2; TBCF10839 pilY1::Tn5 mutant 25C8, lane 3; TBCF10839 pilY1::Tn5 mutant 10CB5, lane 4; TBCF10839 pilY1::Tn5 mutant 25C8 complemented with pME6010::TBpilY1, lane 5; and TBCF10839 pilW::Tn5 mutant 14D1, lane 6; grown until late exponential phase. Equivalent amount from cell lysates were subjected to 6% SDS-PAGE followed by immunodetection with rabbit polyclonal anti-PilY1 antibody (Eurogentec). The asterisk indicates the immunoreactive band of a truncated PilY1 protein in mutant 10CB5. Lower panel: Sensitivity of the rabbit polyclonal anti-PilY1 antibody against the PilY1-specific oligopeptide used for immunization.