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. 2008 Nov 6;71(1):48–65. doi: 10.1111/j.1365-2958.2008.06508.x

Fig. 5.

Fig. 5

Western blot of saponin pellets of trophozoite-infected erythrocytes with CS2, CS2Pf332trunc and CS2ΔPf332 using monoclonal antibody 10H2 that is specific for the Pf332 DBL domain. Time-course across the asexual blood stage with equal number of parasites added to each lane showing maximal expression of Pf332 full length and the Pf332 truncation in trophozoite stages. Parasite pellets from synchronized CS2 parasites taken at 8 h intervals were separated by SDS-PAGE, transferred to nitrocellulose and probed with the mouse anti-Pf332 10H2 monoclonal antibody. Anti-PfHSP70 antibodies shown at the bottom of each panel was used to confirm correct development of the parasites across the asexual blood stage.