Fig. 2. Δicp lacks CP inhibitory activity.

A. T. cruzi epimastigote lysates and T. brucei WT BSF lysates were boiled and tested (8.5 μg protein ml⁻¹) for inhibitory CP activity by pre-incubation with 3 nM papain for 20 min. The residual activity of the enzyme was measured using 15 μM Z-Phe-Arg-MCA. B. BSF lysates (50 μg protein) were boiled and tested for the inhibition of papain by pre-incubating with 2 nM papain for 20 min, followed by determination of residual peptidase activity using 5 μM of Z-Phe-Arg-MCA. Asterisk shows scores statistically significant from buffer at P < 0.05.