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. Author manuscript; available in PMC: 2009 May 11.
Published in final edited form as: J Biol Chem. 2007 Jun 26;282(35):25640–25648. doi: 10.1074/jbc.M701129200

FIGURE 4. Binding of PMCA4a to TA-CaM.

FIGURE 4

A: Changes of fluorescence in TA-CaM upon mixing with microsomes containing 50 nM of either PMCA4a or PMCA4b. Inset: Traces of the first 5 s of the reaction. B: Changes of fluorescence in TA-CaM upon mixing with microsomes containing the concentration of PMCA4a indicated in the figure. The experimental data are shown in grey. The fit to the model depicted in C is shown in continuous black lines. TA-CaM concentration was 17 nM in all cases. C: Kinetic model that represents the binding of CaM to PMCA4a.