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. Author manuscript; available in PMC: 2009 May 11.
Published in final edited form as: J Biol Chem. 2007 Jun 26;282(35):25640–25648. doi: 10.1074/jbc.M701129200

FIGURE 7. Dissociation of peptides C28-4a, C28-4b, C28-4aD/K and C28-4bK/D from TA-CaM.

FIGURE 7

A: TA-CaM was pre-mixed with the peptide indicated and dissociation was then initiated by mixing with unlabeled CaM. Final concentrations: TA-CaM, 17 nM; peptide, 200 nM; CaM, 1 µM. All other conditions were as described in Materials and Methods. B: Comparison of the dissociation rates (koff) of the peptides from TA-CaM as determined from the data in panel A. Note that most of the difference in dissociation rates between C28-4a and C28-4b appears to be due to the difference in a single charged residue (D in 4a and K in 4b) as illustrated by the dissociation rates of the reciprocal mutant peptides C28-4aD/K and C28-4bK/D.