Protein separation by cross-pressed and 4-way compressed tube assembly.
Separation column: 1. 35 mm ID PTFE cross-pressed spiral tube consisting of 15 spiral layers with 85 ml capacity. It was compressed along four radial grooves to improve the partition efficiency; sample, solvent system and other conditions: see Fig. 4 caption. In L-I-T and L-I-H, the first peaks represent myoglobin and the second peaks, lysozyme while in U-O-T and U-O-H, the first peaks represent lysozyme and the second peaks, myoglobin.