. Author manuscript; available in PMC: 2010 May 8.

Published in final edited form as: J Chromatogr A. 2008 Nov 13;1216(19):4193–4200. doi: 10.1016/j.chroma.2008.10.126

Table 1.

Experimental conditions and separation efficiencies of proteins with 1.6 mm ID PTFE cross-pressed coil with 95 ml capacity at 800 rpm

Elution mode	Flow rate (ml/min)	T P (1st/2nd peaks)	Rs	S_F (%)
L-I-T (CCW)	0.5	24/29	1.53	75.7
	1	31/31	1.10	66.3
	2	55/53	1.39	54.0
L-I-H (CW)	0.5	33/39	1.23	65.0
	1	35/22	0.84	63.5
	2	32/21	0.96	50.6
U-O-T (CW)	0.5	41/34	1.20	71.9
	1	31/43	1.05	60.6
	2	43/15	0.59	31.2
U-O-H (CCW)	0.5	57/46	1.44	73.0
	1	73/52	1.40	63.5
	2	137/72	1.74	49.4

L-I-T: lower mobile phase pumped into the inner tail terminal; L-I-H: lower mobile phase pumped into the inner head terminal; U-O-T: upper mobile phase pumped into the outer tail terminal; U-O-H: upper mobile phase pumped into the outer head terminal; direction of rotation viewing the spiral disk :CCW, counterclockwise rotation; CW, clockwise rotation; TP: theoretical plate number; R_S: peak resolution; S_F(%); % stationary phase retention; 1^st peak: myoglobin in L-I-T or L-I-H and lysozyme in U-O-T or U-O-H; 2^nd peak: lysozyme in L-I-T or L-I-H and myoglobin in U-O-T or U-O-H.