. Author manuscript; available in PMC: 2010 May 8.

Published in final edited form as: J Chromatogr A. 2008 Nov 13;1216(19):4193–4200. doi: 10.1016/j.chroma.2008.10.126

Table 3.

Experimental conditions and separation efficiencies of proteins with 1.6 mm ID PTFE tubing compressed at 4 radial grooves at 2 ml/min flow rate

Elution mode	rpm	TP (1st/2nd peaks)	Rs	S_F (%)
L-I-T (CCW)	600	50/42	0.86	54.2
	800	81/57	1.23	52.4
	1000	147/138	1.96	67.8
	1200	212/146	2.25	67.2
U-O-H (CCW)	600	132/37	0.72	33.9
	800	144/67	1.48	58.3
	1000	247/121	1.85	64.2
	1200	441/150	2.53	73.0

L-I-T: lower mobile phase pumped into the inner tail terminal; U-O-H: upper mobile phase pumped into the outer head terminal; direction of rotation viewing the spiral disk :CCW, counterclockwise rotation; TP: theoretical plate number; R_S: peak resolution; S_F(%); % stationary phase retention; 1^st peak: myoglobin in L-I-T and lysozyme in U-O-H; 2^nd peak: lysozyme in L-I-T and myoglobin in U-O-H.