Table 5.
Separation of proteins by a spiral tube assembly with cross-pressed and 4-way compressed 1.35 mm ID PTFE tube with 15 spiral layers and a total capacity of 85 ml.
| Flow | 0.5 ml/min | 1 ml/min | 2 ml/min | ||||||
|---|---|---|---|---|---|---|---|---|---|
| rpm | TP | Rs | SF | TP | Rs | SF | TP | Rs | SF |
| 600 | 43/54(35/28* | 1.62 (1.04) | 55.9(63.0) | 72/50(56/30) | 1.04(1.13) | 39.4(54.3) | 144/46(46/25) | 0.82(0.58) | 21.2(19.7) |
| 800 | 96/66(27/32) | 1.75 (1.27) | 63.2(76.6) | 86/80(44/44) | 1.50(1.24) | 50.0(61.5) | 144/104(128/66) | 1.18(1.11) | 35.3(44.2) |
| 1000 | 51/76(40/91) | 1.90 (1.61) | 70.6(76.0) | 85/104(77/90) | 1.88 (1/78) | 55.9(71.2) | 228/275(88/138) | 2.18(1.81) | 41.2(56.7) |
| 1200 | 68/91 | 2.16 | 70.6 | 144/168(92/122) | 2.61 (2.29) | 67.1(71.2) | 455/249(133/191) | 2.64(2.06) | 47.1(59.6) |
TP: theoretical plate numbers for the first / second peaks; **Rs: Peak resolution; SF: % retention of the stationary phase; elution modea: L-I-T
*
Figures in parentheses indicate the date from 4-way compressed plain tube with 15 layers and 120 ml capacity.