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. Author manuscript; available in PMC: 2010 May 1.

Published in final edited form as: Mol Carcinog. 2009 May;48(5):454–464. doi: 10.1002/mc.20483

Fig. 3 — GADD45α1 is unable to sensitize As³⁺ induced mitotic arrest. A. The BEAS-2B cells stably expressing a control vector, GADD45α (WT) or GADD45α1 (ΔE2) were cultured in the absence or presence of 20 μM As³⁺ for 12 h. The cell mitosis was indicated by immunofluorescence staining using anti-phospho-histone H3 antibody (p-H3) and counter stained with DAPI to visualize the nuclei. B. Average percentage of the p-H3 positive cells of the vector-, GADD45α (wt)- or GADD45α1 (ΔE2)-transfected cells cultured in the absence or presence of 20 μM A³⁺ for 12 h. Data are means ± SD (n=3). *: p < 0.01; **:p < 0.005. C. Western-blotting analysis of the p-H3 in the cells transfected with the indicated vectors and cultured in the absence or presence of As³⁺. D. Morphological analysis suggested that As³⁺ treatment arrests cells in prometaphase.

Fig. 3 — GADD45α1 is unable to sensitize As³⁺ induced mitotic arrest. A. The BEAS-2B cells stably expressing a control vector, GADD45α (WT) or GADD45α1 (ΔE2) were cultured in the absence or presence of 20 μM As³⁺ for 12 h. The cell mitosis was indicated by immunofluorescence staining using anti-phospho-histone H3 antibody (p-H3) and counter stained with DAPI to visualize the nuclei. B. Average percentage of the p-H3 positive cells of the vector-, GADD45α (wt)- or GADD45α1 (ΔE2)-transfected cells cultured in the absence or presence of 20 μM A³⁺ for 12 h. Data are means ± SD (n=3). *: p < 0.01; **:p < 0.005. C. Western-blotting analysis of the p-H3 in the cells transfected with the indicated vectors and cultured in the absence or presence of As³⁺. D. Morphological analysis suggested that As³⁺ treatment arrests cells in prometaphase.