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. Author manuscript; available in PMC: 2009 May 11.
Published in final edited form as: J Cell Biochem. 2007 Feb 15;100(3):617–628. doi: 10.1002/jcb.21087

Fig. 1.

Fig. 1

The effect of 1,25(OH)2D3 on endogenous VDR in Caco-2 and ROS 17/2.8 cells. (A) ROS 17/2.8 and parental Caco-2 cells were treated with either 100 nM 1,25(OH)2D3 (1,25 VD) or vehicle (EtOH) for 1 h and VDR protein levels were determined in whole cell extracts by Western blot analysis. (B) Caco-2 cells were treated with either 10 nM 1,25(OH)2D3 (1,25 VD) or vehicle (EtOH) for 2 h. Whole cell and nuclear extracts were examined for VDR protein levels by Western blot analysis. The figures are representative blots of individual samples from experiments with n=3 replicates; experiments were conducted three times.