Fig. 3.
Calcitriol causes significant changes in distribution of GFP-VDR in ROS 17/2.8 but not in BBe cells. The images of proliferating BBe cells or ROS17/2.8 (A1G) cells were taken before (baseline) and 30 min after incubation with 100 nM 1,25(OH)2D3. (A) Representative confocal images. (B) Image analysis of n=6 cells per cell type and treatment. The change in % signal intensity was calculated as described in Materials and Methods Section. Data represent mean±SE. Values with different superscripts are significantly different from one another (P<0.05, n=6 cells per treatment).