Calcitriol stimulates rapid translocation of GFP-VDR to the nucleus of BBe cells. A: Representative time series for a single differentiated BBe cell response to 100 nM 1,25(OH)2D3 (1,25 VD) or vehicle (EtOH), (B) summary of the response to 1,25(OH)2D3 in proliferating BBe cells, (C) summary of the response to 1,25(OH)2D3 in differentiated BBe cells. Nuclear photobleaching of GFP-VDR transfected cells was conducted at t=0, a baseline image was collected and immediately afterwards cells were treated with either 100 nM 1,25(OH)2D3 (1,25 VD) or vehicle (EtOH). Images of cells were taken at 2.5, 5, and every 5 min until 30 min. Points represent the mean±SEM of n=6 cells per treatment. * Values for a time point are significantly different between vehicle and vitamin D treated cells, P<0.05.