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. Author manuscript; available in PMC: 2009 May 11.
Published in final edited form as: Science. 2008 Oct 10;322(5899):268–271. doi: 10.1126/science.1164164

Figure 2. Pfn and GrB are required to maintain HSV-1 neuronal latency in vivo and in ex vivo ganglia cultures.

Figure 2

(A) DNA was extracted from individual TG at designated times and HSV-1 genome copies was determined by quantitative real-time PCR (horizontal bar = mean). Data pooled from at least two independent experiments per time point. * p≤0.05 as calculated by ANOVA with Bonferroni post-test. (B) 34–41 dpi TG were dispersed and cultured at one-fifth TG equivalents per well. HSV-1 reactivation was indicated by the presence of infectious virus in serially sampled supernatants as assessed by plaque assay. Pooled data from three independent experiments presented as mean ± SEM. * p=0.0009; ** p=0.0002 as calculated by survival curve analysis (Log-rank test). (C) 14 dpi TG were dispersed and cultured at 1 TG per well in medium alone or in medium supplemented with 1000 U/ml recombinant IFN-γ (rIFN-γ), 100 μg/ml anti-CD8α monoclonal antibody (α-CD8α mAb), or 20 μg/ml anti-IFN-γ monoclonal antibody (α-IFN-γ mAb). Reactivation was assessed, analyzed, and presented as in (B). n=10 TG/condition. Data representative of two independent experiments. * p=0.0108; ** p=0.0049.