Fig. 2.

The initiation of curved growth in C. albicans hyphae is affected by substrate rigidity (A) or the availability of a carbon source (B). Hyphae were grown on 5% or 20% (v/v) serum and concentrations of agar varied from 1% to 6% (A). Hyphae were grown on 20% (v/v) serum ± 2% (w/v) glucose, maltose or sorbitol at 37°C for 10 h (B). In both experiments, hyphae were viewed by light microscopy and the number of primary hyphae over 80 μm in length that formed two or more consecutive sinusoidal curves was expressed as a percentage of all hyphae formed (error bars are means ± SD, n = 100 hyphae from three independent experiments).