Fig. 3.

The effect of varying calcium availability and deletion of calcium-related genes on the initiation of sinusoidal growth (A). Hyphal growth was induced on solid agar medium at 37°C and the number of primary hyphae forming two or more consecutive regular sinusoidal waves was determined (error bars are means ± SD, n = 100 hyphae from three independent experiments). Light microscopy of hyphae formed by the control strain (B, upper panel) and the pmr1Δ mutant (B, lower panel) during growth on 20% (v/v) serum solidified with 4% agar. Bars = 10 µm.