Zinc activates NF-κB. Cultures were transfected with a GFP reporter gene driven by an NF-κB promoter. A, Photomicrographs show microglial morphology (left) and GFP expression (right) in cultures treated with 0 or 15 μm zinc for 24 h. B, Quantified evaluation of NF-κB expression in microglia, assessed at 2 or 24 h after incubation with ZnCl2. The positive control, TNFα, indicates the fraction of microglia successfully transfected with the GFP reporter gene. *p < 0.05 versus control; n = 3. Error bars indicate SEM.