Figure 5.
Zinc increases nitric oxide synthase (NOS) activation and F4/80 expression. A, Nitrite was measured 24 h after 90 min incubations under the designated conditions. ZnCl2, 30 μm; aminoguanidine (AG), 1 mm; PJ-34, 400 nm; LPS, 50 ng/ml. n = 4; *p < 0.05. B, Western blot shows F4/80 expression at 160 kDa (top) and β-actin loading control (bottom). The vertical lines denote independently prepared blots. Cultures were prepared from wild-type, PARP-1−/−, or p47phox−/− microglia, after treatment as denoted on the bar graph. Abbreviations are as in A. Graph indicates quantified data from n = 3, normalized to expression in control, wild-type cells. *p < 0.05 versus wild-type control. Error bars indicate SEM.