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Neuroprotective effect of bilirubin on H2O2-induced toxicity. (A) Increasing concentrations of free BR were added to rat primary hippocampal neurons before addition of 75 μM H2O2. (B) Increasing concentrations of BR complexed with human serum albumin (BR–HSA was added to neurons). An equivalent concentration HSA of albumin was added. Neuron survival was estimated 24 h after the beginning of the experiment.
