Table 1.
Included metabolites
| No. | [MH+] | RT | Metabolite | R2 | RSD [%] | Symbol | Metabolic pathway# |
|---|---|---|---|---|---|---|---|
| 1 | 302 | 3.0 | 1-ribosyl-3-methyl-5-(2-aminocarboxyethyl)-imidazolium | 0.9875 | 7.4 | M-1 | (H) |
| 2 | 247 | 4.3 | Dihydrouridine | 0.9855 | 6.0 | DHU | R |
| 3 | 245 | 4.5 | Pseudouridine | 0.9889 | 3.3 | Ψ | R |
| 4 | 212 | 5.0 | 1-ribosyl-pyridinium | 0.9771 | 5.7 | M-2 | (N) |
| 5 | 244 | 6.5 | Cytidine | 0.9815 | 12.0 | C | R |
| 6 | 346 | 7.0 | 3-(3-aminocarboxypropyl)-uridine | 0.9957 | 3.5 | acp3U | R |
| 7 | 302 | 7.0 | 5-carbamoylmethyluridine | 0.9977 | 4.0 | ncm5U | R |
| 8 | 228 | 7.5 | 1-ribosyl-3-hydroxy-pyridinium | 0.9932 | 2.1 | M-3 | (N) |
| 9 | 245 | 8.5 | Uridine | 0.9981 | 8.4 | U | R |
| 10 | 258 | 9.5 | 3-methylcytidine | 0.9787 | 2.7 | m3C | R |
| 11 | 259 | 10.5 | 1-ribosyl-4-carbamoyl-5-amino-imidazole | 0.9942 | 6.0 | AICA riboside | N |
| 12 | 282 | 12.0 | 1-methyladenosine | 0.9949 | 3.1 | m1A | R |
| 13 | 271 | 14.0 | 1-ribosyl-5-carbamoyl-2-oxo-pyridine | 0.9903 | 3.7 | 2,5-PCNR | N |
| 14 | 298 | 17.5 | 7-methylguanosine | 0.9727 | 10.2 | m7G | R |
| 15 | 269 | 18.0 | Inosine | 0.9911 | 3.0 | I | R |
| 16 | 271 | 20.5 | 1-ribosyl-3-carbamoyl-4-oxo-pyridine | 0.9955 | 2.1 | 3,4-PCNR | N |
| 17 | 296 | 21.5 | 1, N6-dimethyladenosine | 0.9959 | 2.9 | m61A | R |
| 18 | 259 | 23.5 | 3-methyluridine | 0.9972 | 3.0 | m3U | R |
| 19 | 384 | 24.5 | N6-succinyloadenosine | 0.9568 | 11.3 | N6-SAR | P |
| 20 | 285 | 25.0 | Xanthosine | 0.9974 | 4.0 | X | R |
| 21 | 385 | 27.5 | S-adenosylhomocysteine | 0.9926 | 4.4 | SAH | MP |
| 22 | 283 | 29.0 | 1-methylinosine | 0.9969 | 3.8 | m1I | R |
| 23 | 298 | 31.0 | 1-methylguanosine | 0.9920 | 4.5 | m1G | R |
| 24 | 293 | 32.0 | ? | 0.9918 | 14.9 | 293 | ? |
| 25 | 286 | 32.5 | N4-acetylcytidine | 0.9934 | 7.6 | ac4C | R |
| 26 | 298 | 33.5 | 2-methylguanosine | 0.9979 | 8.2 | m2G | R |
| 27 | 376 | 34.5 | 9-ribosyl-6-taurinopurine | 0.9974 | 6.2 | M-4 | (R) |
| 28 | 398 | 40.0 | 2-methylthio-N6-(cis-hydroxyisopentenyl)-adenosine | 0.9983 | 4.5 | ms2io6A | R |
| 29 | 326 | 41.0 | N2, N2,7-trimethylguanosine | 0.9911 | 2.7 | m2,2,7G | R |
| 30 | 312 | 41.5 | N2, N2-dimethylguanosine | 0.9925 | 4.7 | m22G | R |
| 31 | 333 | 42.5 | 5-methoxycarbonylmethyl-2-thiouridine | 0.9981 | 4.6 | mcm5s2U | R |
| 32 | 413 | 46.5 | N6-threonylcarbamoyladenosine | 0.9963 | 3.5 | t6A | R |
| 33 | 298 | 48.0 | 5'-deoxy-5'-methyl-thioadenosine | 0.9987 | 13.3 | MTA | MP |
| 34 | 427 | 49.5 | N6-methyl-N6-threonylcarbamoyladenosine | 0.9920 | 5.5 | m6t6A | R |
| 35 | 459 | 50.0 | 2-methylthio-N6-threonylcarbamoyladenosine | 0.9887 | 3.9 | ms2t6A | R |
Set of included ribosylated metabolites for bioinformatic data evaluation.
# R: RNA metabolism, H: histidine metabolism, N: nicotinate/nicotinamide metabolism,
MP: methionine/polyamin cycle, P: purine biosynthesis, ?: unknown. Abbreviations in parenthesis: pathway proposal, RT: retention time. (Structures M-1 to M-4 see Figure 1.)